Bone tissue engineering using P-15 coated scaffolds and human dental pulp stromal cells
نویسندگان
چکیده
Introduction P-15 a fifteen residue synthetic peptide (GTPGPQGIAGQRGVV), is a structural analogue of the cell binding domain of Type 1 collagen [1]. P-15 adsorbed on anorganic bovine mineral (ABM-P15) scaffolds has been shown to enhance bone marrow stromal cell growth [2]. This study aimed to investigate the osteogenic potential of human dental pulp stromal cells (HDPSCs) in monolayer culture compared to human bone marrow stromal cells (HBMSCs) and on 3D ABM-P15 in vitro and in vivo.
منابع مشابه
Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass® Scaffolds
Identification of a suitable cell source combined with an appropriate 3D scaffold is an essential prerequisite for successful engineering of skeletal tissues. Both osteogenesis and angiogenesis are key processes for bone regeneration. This study investigated the vascularization potential of a novel combination of human dental pulp stromal cells (HDPSCs) with 45S5 Bioglass® scaffolds for tissue-...
متن کاملRegeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds
BACKGROUND The aim of this study was to characterize the efficacy of nano-hydroxyapatite-coated silk fibroin constructs as a scaffold for bone tissue engineering and to determine the osteogenic effect of human dental pulp and periodontal ligament derived cells at an early stage of healing in rabbits. 3D silk fibroin constructs were developed and coated using nano-hydroxyapatite crystals. Dental...
متن کاملPrinciples and methods of dental nerve tissue regeneration : a review
Background and Aim Today, tissue engineering is considered a significant approach in modern medicine, which is why research in biomaterials has focused on the development of advanced scaffolding for regenerative medicine. Many natural and synthetic polymers with a variety of origins have been used to make these scaffolds or are recommended by researches. These compositions usually have the pr...
متن کاملسلولهای بنیادین پالپ دندانهای شیری انسان، تاریخچه و انواع روشهای استخراج سلول
Background and Aims: In the last decade, several studies have reported the isolation of stem cell population from different dental sources, while their mesenchymal nature is still controversial. The aim of this study was to introduce the isolating methods for stem cells from human dental pulp and to determine their mesenchymal nature before differentiation. Material and methods: One of the ...
متن کاملPerspectives of chitosan nanofiber/film scaffolds with bone marrow stromal cells in tissue engineering and wound dressing
Objective (s): Several methods have been proposed for repairing defects and damages, one of which is cell therapy. Bone marrow stromal cells seem to be suitable for this purpose. On the other hand, many biometric materials are used to improve and correct the defects in the body. Nanofibers are widely used in the medical industry, especially in tissue engineering, as scaffolds in wound healing a...
متن کامل